The newest part out of Caenorhabditis elegans intercourse-determination homologs, Mi-sdc-1 and you can Mi-tra-one in Meloidogyne incognita
Doing a novel?
Gender dedication is a button developmental enjoy in all bacteria. The brand new pathway one to regulates intimate destiny has been well characterized on brand new unit height about model free-way of living nematode Caenorhabditis elegans. This study will gain an initial comprehension of gender-deciding paths when you look at the a herb-parasitic nematode Meloidogyne incognita, and the quantity that the fresh new spots of your intercourse commitment family genes is actually stored for the a hermaphrodite variety, C. elegans, and you can bush-parasitic nematode variety, Yards. incognita. Within study, we targeted two sex-choosing orthologues, sdc-1 and tra-step 1 out-of M. incognita playing with RNA interference (RNAi). RNAi is actually did by drenching next-stage juveniles regarding M. incognita inside the an answer containing dsRNA regarding possibly Mi-tra-1or Mi-sdc-1 otherwise both. To search for the aftereffect of RNAi of the address genetics, the latest juveniles addressed with the brand new dsRNA was indeed inoculated on to a susceptible cultivar away from cowpea mature into the a nutrient pouch at the twenty eight °C for five weeks. Lire la suite